High-level fluoroquinolone resistance in Streptococcus pneumoniae requires mutations in parC and gyrA
نویسندگان
چکیده
منابع مشابه
Antimicrobial Susceptibility Breakpoints and First-Step parC Mutations in Streptococcus pneumoniae: Redefining Fluoroquinolone Resistance
Clinical antimicrobial susceptibility breakpoints are used to predict the clinical outcome of antimicrobial treatment. In contrast, microbiologic breakpoints are used to identify isolates that may be categorized as susceptible when applying clinical breakpoints but harbor resistance mechanisms that result in their reduced susceptibility to the agent being tested. Currently, the National Committ...
متن کاملHorizontal transfer of parC and gyrA in fluoroquinolone-resistant clinical isolates of Streptococcus pneumoniae.
We have analyzed genetically three clinical isolates (3180, 3870, and 1244) of Streptococcus pneumoniae with high-level ciprofloxacin resistance. Isolates 3180 and 3870 were atypical because of their insolubility in deoxycholate. However, they hybridized specifically with pneumococcal autolysin and pneumolysin gene probes and have typical pneumococcal atpC and atpA gene sequences. Analysis of t...
متن کاملAn extended PCR-RFLP assay for detection of parC, parE and gyrA mutations in fluoroquinolone-resistant Streptococcus pneumoniae.
Sir, In Gram-positive cocci, fluoroquinolone resistance is associated with mutational alterations in DNA gyrase and/or topoisomerase IV or with active efflux of the drugs. In Streptococcus pneumoniae, fluoroquinolone resistance is mainly due to substitutions in ParC (Ser-79→Phe or Tyr, Asp-83→Asn), ParE (Asp-435→Asn) or GyrA (Ser-81→Phe or Tyr, Glu-85→Lys or Gly).1,2 Therefore, detection of the...
متن کاملAmino acid Substitution Mutations Analysis of gyrA and parC Genes in Clonal Lineage of Klebsiella pneumoniae conferring High-Level Quinolone Resistance
Background: Emergence Klebsiella pneumoniae resistant to quinolone antibiotics due to mutations in gyrA and parC genes created problem for treatment of patients in different hospitals in Iran. The objective of this study was to determine the amino acid substitutions of GyrA and ParC proteins in certain clonal lineages of the K. pneumoniae conferring high level quinolone resistance. Methods: One...
متن کاملFluoroquinolone Resistance Linked to GyrA, GyrB, and ParC Mutations in Salmonella enterica Typhimurium Isolates in Humans
We report two cases of infection with clonally unrelated, high-level ciprofloxacin-resistant, b-lactamase-producing strains of Salmonella enterica Typhimurium. Resistance was caused by four topoisomerase mutations, in GyrA, GyrB, and ParC and increased drug efflux. Ciprofloxacin treatment failed in one case. In the second case, reduced susceptibility to third-generation cephalosporins occurred ...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1996
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.40.12.2760